Publication list of Alexander K. Buell
2024
A Farzadfard, A Kunka, TO Mason, JA Larsen, RK Norrild, ET Dominguez, S Ray, AK Buell Thermodynamic characterization of amyloid polymorphism by microfluidic transient incomplete separation . CHEMICAL SCIENCE, JAN 8, 2024. DOI: 10.1039/D3SC05371G
2023
RK Norrild, TO Mason, L Boyens-Thiele, S Ray, JB Mortensen, AW Fritsch, JM Iglesias-Artola, LK Klausen, EGP Stender, H Jensen, AK Buell Taylor-dispersion induced phase separation for the efficient characterisation of protein condensate formation . CHEMRXIV, JUL 14, 2023. DOI: 10.26434/chemrxiv-2023-1ztrt
S Ray, TO Mason, L Boyens-Thiele, A Farzadfard, JA Larsen, RK Norrild, N Jahnke, AK Buell Mass photometric detection and quantification of nanoscale α-synuclein phase separation . NATURE CHEMISTRY, JUN 19, 2023. DOI: 10.1038/s41557-023-01244-8
S Nath, AK Buell, B Barz Pyroglutamate-modified Amyloid β (3-42) Monomer has more β-sheet content than the Amyloid β (1-42) Monomer . PHYS. CHEM. CHEM. PHYS., JUN, 2023. DOI: 10.1039/D2CP05961D
L Zhai, Z Wang, F Liu, C Xu, J Wang, H Han, Q Xie, W Zhang, Y Zheng, AK Buell, Y Dong Semi-rational evolution of a recombinant DNA polymerase for modified nucleotide incorporation efficiency . BIORXIV, MAR, 2023. DOI: 10.1101/2023.03.20.533374
C Galvagnion, A Barclay, K Makasewicz, FR Marlet, M Moulin, J Devos, S Linse, A Martel, L Porcar, E Sparr, M Cramer Pedersen, F Roosen-Runge, L Arleth, AK Büll Structural characterisation of α-synuclein-membrane interactions and the resulting aggregation using small angle scattering . CHEMRXIV, MAR, 2023. DOI: 10.26434/chemrxiv-2023-6hsh2
J Shenoy, A Lends, M Berbon, M Bilal, N El Mammeri, M Bertoni, A Saad, E Morvan, A Grélard, S Lecomte, F Theillet, AK Buell, B Kauffmann, B Habenstein, A Loquet Structural polymorphism of the low-complexity C-terminal domain of TDP-43 amyloid aggregates revealed by solid-state NMR . FRONT. MOL. BIOSCI., MAR, 2023. DOI: 10.3389/fmolb.2023.1148302
H Mohammad-Beigi, W Wijaya, M Madsen, Y Hayashi, R Li, TAM Rovers, TC Jæger, AK Buell, AB Hougaard, JJK Kirkensgaard, P Westh, R Ipsen, B Svensson Association of caseins with β-lactoglobulin influenced by temperature and calcium ions: A multi-parameter analysis . FOOD HYDROCOLLOIDS, 2023. DOI: 10.1016/j.foodhyd.2022.108373
AV Madsen, P Kristensen, AK Buell, S Goletz Generation of robust bispecific antibodies through fusion of single-domain antibodies on IgG scaffolds: a comprehensive comparison of formats . MABS, MAR, 2023. DOI: 10.1080/19420862.2023.2189432
R Sternke-Hoffmann, T Pauly, RK Norrild, J Hansen, F Tucholski, M Haraldson Høie, P Marcatili, M Dupré, M Duchateau, M Rey, C Malosse, S Metzger, A Boquoi, F Platten, SU Egelhaaf, J Chamot-Rooke, R Fenk, L Nagel-Steger, R Haas, AK Buell Widespread amyloidogenicity potential of multiple myeloma patient-derived immunoglobulin light chains . BMC BIOLOGY, FEB, 2023. DOI: 10.1186/s12915-022-01506-w
2022
H Mohammad-Beigi, W Wijaya, M Madsen, Y Hayashi, R Li, TAM Rovers, TC Jæger, AK Buell, AB Hougaard, JJK Kirkensgaard, P Westh, R Ipsen, B Svensson. Association of caseins with β-lactoglobulin influenced by temperature and calcium ions: A multi-parameter analysis. BIORXIV, DEC 7, 2022. DOI: 10.1016/j.foodhyd.2022.108373
J Bartl, M Zanini, F Bernardi, A Forget, L Blumel, J Talbot, D Picard, N Qin, G Cancila, QS Gao, S Nath, IM Koumba, M Wolter, F Kuonen, M Langini, T Beez, C Munoz, D Pauck, V Marquardt, H Yu, J Souphron, M Korsch, C Molders, D Berger, S Gobbels, FD Meyer, B Scheffler, B Rotblat, S Diederichs, V Ramaswamy, H Suzuki, A Oro, K Stuhler, A Stefanski, U Fischer, G Leprivier, D Willbold, G Steger, A Buell, M Kool, P Lichter, SM Pfister, PA Northcott, MD Taylor, A Borkhardt, G Reifenberger, O Ayrault, M Remke. The HHIP-AS1 lncRNA promotes tumorigenicity through stabilization of dynein complex 1 in human SHH-driven tumors . NATURE COMMUNICATIONS, JUL 13, 2022. DOI: 10.1038/s41467-022-31574-z
S Ray, TO Mason, L Boyens-Thiele, N Jahnke, AK Buell. Mass photometric detection and quantification of nanoscale α-synuclein phase separation. BIORXIV, MAY 4, 2022. DOI: 10.1101/2022.05.03.490467
A Farzadfard, A König, SV Petersen, J Nielsen, E Vasili, A Dominguez-Meijide, AK Buell, TF Outeiro, DE Otzen. Glycation modulates alpha-synuclein fibrillization kinetics: A sweet spot for inhibition. JBC, MAY, 2022. DOI: 10.1016/j.jbc.2022.101848
VL Leguizamon Herrera, AK Buell, D Willbold, B Barz. Interaction of Therapeutic d-Peptides with Aβ42 Monomers, Thermodynamics, and Binding Analysis. ACS CHEMICAL NEUROSCIENCE, MAY 17, 2022. DOI: 10.1021/acschemneuro.2c00102
D Zhang, YQ Sheng, N Piano, T Jakuszeit, EJ Cozens, LQ Dong, AK Buell, A Pollet, IM Lei, WY Wang, E Terentjev, YYS Huang. Cancer cell migration on straight, wavy, loop and grid microfibre patterns. BIOFABRICATION, APR 1, 2022. DOI: 10.1088/1758-5090/ac48e6
AK Buell. Stability matters, too – The thermodynamics of amyloid fibril formation. Chem. Sci., 2022. DOI: 10.1039/D1SC06782F
H Mohammad-Beigi, M Zanganeh, C Scavenius, H Eskandari, A Farzadfard, SA Shojaosadati, JJ Enghild, DE Otzen, AK Buell, DS Sutherland. A Protein Corona Modulates Interactions of α-Synuclein with Nanoparticles and Alters the Rates of the Microscopic Steps of Amyloid Formation. ACS NANO, JAN 4, 2022. DOI: 10.1021/acsnano.1c08825
2021
EGP Stender, S Ray, RK Norrild, JA Larsen, D Petersen, A Farzadfard, C Galvagnion, H Jensen, AK Buell. Capillary flow experiments for thermodynamic and kinetic characterization of protein liquid-liquid phase separation. NATURE COMMUNICATIONS, DEC 15, 2021. DOI: 10.1038/s41467-021-27433-y
S Nath, P Roy, R Mandal, R Roy, AK Buell, N Sengupta, PK Tarafdar. Hydroxy-Porphyrin as an Effective, Endogenous Molecular Clamp during Early Stages of Amyloid Fibrillization. CHEMISTRY-AN ASIAN JOURNAL, DEC 1, 2021. DOI: 10.1002/asia.202100965
DE Otzen, AK Buell, H Jensen. Microfluidics and the quantification of biomolecular interactions. CURRENT OPINION IN STRUCTURAL BIOLOGY, OCT, 2021. DOI: 10.1016/j.sbi.2021.02.006
M Dupre, M Duchateau, R Sternke-Hoffmann, A Boquoi, C Malosse, R Fenk, R Haas, AK Buell, M Rey, J Chamot-Rooke. De Novo Sequencing of Antibody Light Chain Proteoforms from Patients with Multiple Myeloma. ANALYTICAL CHEMISTRY, AUG 3, 2021. DOI: 10.1021/acs.analchem.1c01955
S Nath, AK Buell, B Barz, L Gremer, SS Linse, D Willbold. Pyroglumate-modified A beta(3-42) monomers bind with higher affinity than A beta 42 monomers to A beta 42 fibrils. EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS, JUL, 2021.
AK Buell. Editorial for the special issue “Protein misfolding and amyloid aggregation” of Biophysical Chemistry. BIOPHYSICAL CHEMISTRY, MAY, 2021. DOI: 10.1016/j.bpc.2021.106566
B Lopez-Mendez, B Baron, CA Brautigam, TA Jowitt, SH Knauer, S Uebel, MA Williams, A Sedivy, O Abian, C Abreu, M Adamczyk, W Bal, S Berger, AK Buell, C Carolis, T Daviter, A Fish, M Garcia-Alai, C Guenther, J Hamacek, J Holkova, J Houser, C Johnson, S Kelly, A Leech, C Mas, D Matulis, SH McLaughlin, R Montserret, R Nasreddine, R Nehme, Q Nguyen, D Ortega-Alarcon, K Perez, K Pirc, G Piszczek, M Podobnik, N Rodrigo, J Rokov-Plavec, S Schaefer, T Sharpe, J Southall, D Staunton, P Tavares, O Vanek, M Weyand, D Wu. Reproducibility and accuracy of microscale thermophoresis in the NanoTemper Monolith: a multi laboratory benchmark study. EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS, MAY, 2021. DOI: 10.1007/s00249-021-01532-6
SH Alijanvand, A Peduzzo, AK Buell. Secondary Nucleation and the Conservation of Structural Characteristics of Amyloid Fibril Strains. FRONTIERS IN MOLECULAR BIOSCIENCES, APR 16, 2021. DOI: 10.3389/fmolb.2021.669994
RK Norrild, N Vettore, A Coden, WF Xue, AK Buell. Thermodynamics of amyloid fibril formation from non-equilibrium experiments of growth and dissociation. BIOPHYSICAL CHEMISTRY, APR, 2021. DOI: 10.1016/j.bpc.2021.106549
ED Agerschou, MP Schutzmann, N Reppert, MM Wordehoff, H Shaykhalishahi, AK Buell, W Hoyer. beta-Turn exchanges in the alpha-synuclein segment 44-TKEG-47 reveal high sequence fidelity requirements of amyloid fibril elongation. BIOPHYSICAL CHEMISTRY, FEB, 2021. DOI: 10.1016/j.bpc.2020.106519
B Barz, AK Buell, S Nath. Compact fibril-like structure of amyloid beta-peptide (1-42) monomers. CHEMICAL COMMUNICATIONS, JAN 21, 2021. DOI: 10.1039/d0cc06607a
2020
JHM vanGils, E vanDijk, A Peduzzo, A Hofmann, N Vettore, MP Schutzmann, G Groth, H Mouhib, DE Otzen, AK Buell, S Abeln. The hydrophobic effect characterises the thermodynamic signature of amyloid fibril growth. PLOS COMPUTATIONAL BIOLOGY, MAY, 2020. DOI: 10.1371/journal.pcbi.1007767
A Peduzzo, S Linse, AK Buell. The Properties of alpha-Synuclein Secondary Nuclei Are Dominated by the Solution Conditions Rather than the Seed Fibril Strain. ACS CHEMICAL NEUROSCIENCE, MAR 18, 2020. DOI: 10.1021/acschemneuro.9b00594
R Sternke-Hoffmann, A Boquoi, DLY Niedenhoff, F Platten, R Fenk, R Haas, AK Buell. Biochemical and biophysical characterisation of immunoglobulin free light chains derived from an initially unbiased population of patients wit h light chain disease. PEERJ, MAR 17, 2020. DOI: 10.7717/peerj.8771
R Sternke-Hoffmann, A Peduzzo, N Bolakhrif, R Haas, AK Buell. The Aggregation Conditions Define Whether EGCG is an Inhibitor or Enhancer of alpha-Synuclein Amyloid Fibril Formation. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, MAR, 2020. DOI: 10.3390/ijms21061995
2019
N Vettore, AK Buell. Thermodynamics of amyloid fibril formation from chemical depolymerization. PHYSICAL CHEMISTRY CHEMICAL PHYSICS, DEC 21, 2019. DOI: 10.1039/c9cp04524d
C Galvagnion, D Topgaard, K Makasewicz, AK Buell, S Linse, E Sparr, CM Dobson. Lipid Dynamics and Phase Transition within alpha-Synuclein Amyloid Fibrils. JOURNAL OF PHYSICAL CHEMISTRY LETTERS, DEC 19, 2019. DOI: 10.1021/acs.jpclett.9b03005
T Sneideris, A Sakalauskas, R Sternke-Hoffmann, A Peduzzo, M Ziaunys, AK Buell, V Smirnovas. The Environment Is a Key Factor in Determining the Anti-Amyloid Efficacy of EGCG. BIOMOLECULES, DEC, 2019. DOI: 10.3390/biom9120855
AK Buell. The growth of amyloid fibrils: rates and mechanisms. BIOCHEMICAL JOURNAL, OCT 15, 2019. DOI: 10.1042/BCJ20160868
ED Agerschou, P Flagmeier, T Saridaki, C Galvagnion, D Komnig, L Heid, V Prasad, H Shaykhalishahi, D Willbold, CM Dobson, A Voigt, B Falkenburger, W Hoyer, AK Buell. An engineered monomer binding-protein for alpha-synuclein efficiently inhibits the proliferation of amyloid fibrils. ELIFE, AUG 21, 2019. DOI: 10.7554/eLife.46112
C Roder, N Vettore, LN Mangels, L Gremer, RB Rayelli, D Willbold, W Hoyer, AK Buell, GF Schroder. Atomic structure of PI3-kinase Sh5 amyloid fibrils by cryo-electron microscopy. NATURE COMMUNICATIONS, AUG 21, 2019. DOI: 10.1038/s41467-019-11320-8
M Falke, J Victor, MM Wordehoff, A Peduzzo, T Zhang, GF Schroder, AK Buell, W Hoyer, M Etzkorn. alpha-Synuclein-derived lipoparticles in the study of alpha-Synuclein amyloid fibril formation. CHEMISTRY AND PHYSICS OF LIPIDS, MAY, 2019. DOI: 10.1016/j.chemphyslip.2019.02.009
TO Mason, AK Buell. The Kinetics, Thermodynamics and Mechanisms of Short Aromatic Peptide Self-Assembly. BIOLOGICAL AND BIO-INSPIRED NANOMATERIALS: PROPERTIES AND ASSEMBLY MECHANISMS, 2019. DOI: 10.1007/978-981-13-9791-2_3
2018
T Ziehm, AK Buell, D Willbold. Role of Hydrophobicity and Charge of Amyloid-Beta Oligomer Eliminating D-Peptides in the Interaction with Amyloid-Beta Monomers. ACS CHEMICAL NEUROSCIENCE, NOV, 2018. DOI: 10.1021/acschemneuro.8b00132
JWP Brown, G Meisl, TPJ Knowles, AK Buell, CM Dobson, C Galvagnion. Kinetic barriers to alpha-synuclein protofilament formation and conversion into mature fibrils. CHEMICAL COMMUNICATIONS, JUL 18, 2018. DOI: 10.1039/c8cc03002b
IM vanderWateren, TPJ Knowles, AK Buell, CM Dobson, C Galvagnion. C-terminal truncation of alpha-synuclein promotes amyloid fibril amplification at physiological pH. CHEMICAL SCIENCE, JUL 7, 2018. DOI: 10.1039/c8sc01109e
SIA Cohen, R Cukalevski, TCT Michaels, A Saric, M Tornquist, M Vendruscolo, CM Dobson, AK Buell, TPJ Knowles, S Linse. Distinct thermodynamic signatures of oligomer generation in the aggregation of the amyloid-beta peptide. NATURE CHEMISTRY, MAY, 2018. DOI: 10.1038/s41557-018-0023-x
HZ Gang, C Galvagnion, G Meisl, T Muller, M Pfammatter, AK Buell, A Levin, CM Dobson, BZ Mu, TPJ Knowles. Microfluidic Diffusion Platform for Characterizing the Sizes of Lipid Vesicles and the Thermodynamics of Protein-Lipid Interactions. ANALYTICAL CHEMISTRY, MAR 6, 2018. DOI: 10.1021/acs.analchem.7b04820
T Viennet, MM Wordehoff, B Uluca, C Poojari, H Shaykhalishahi, D Willbold, B Strodel, H Heise, AK Buell, W Hoyer, M Etzkorn. Structural insights from lipid-bilayer nanodiscs link alpha-Synuclein membrane-binding modes to amyloid fibril formation. COMMUNICATIONS BIOLOGY, 2018. DOI: 10.1038/s42003-018-0049-z
2017
TO Mason, TCT Michaels, A Levin, CM Dobson, E Gazit, TPJ Knowles, AK Buell. Thermodynamics of Polypeptide Supramolecular Assembly in the Short-Chain Limit. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, NOV 15, 2017. DOI: 10.1021/jacs.7b00229
MM Wordehoffl, H Shaykhalishahi, L Gross, L Gremer, M Stoldt, AK Buell, D Willbold, W Hoyer. Opposed Effects of Dityrosine Formation in Soluble and Aggregated alpha-Synuclein on Fibril Growth. JOURNAL OF MOLECULAR BIOLOGY, OCT 13, 2017. DOI: 10.1016/j.jmb.2017.09.005
G Meisl, L Rajah, SAI Cohen, M Pfammatter, A Saric, E Hellstrand, AK Buell, A Aguzzi, S Linse, M Vendruscolo, CM Dobson, TPJ Knowles. Scaling behaviour and rate-determining steps in filamentous self-assembly. CHEMICAL SCIENCE, OCT 1, 2017. DOI: 10.1039/c7sc01965c
E Fisher, YY Zhao, R Richardson, M Janik, AK Buell, FI Aigbirhio, G Toth. Detection and Characterization of Small Molecule Interactions with Fibrillar Protein Aggregates Using Microscale Thermophoresis. ACS CHEMICAL NEUROSCIENCE, SEP, 2017. DOI: 10.1021/acschemneuro.7b00228
M Iljina, L Hong, MH Horrocks, MH Ludtmann, ML Choi, CD Hughes, FS Ruggeri, T Guilliams, AK Buell, JE Lee, S Gandhi, SF Lee, CE Bryant, M Vendruscolo, TPJ Knowles, CM Dobson, E DeGenst, D Klenerman. Nanobodies raised against monomeric alpha-synuclein inhibit fibril formation and destabilize toxic oligomeric species. BMC BIOLOGY, JUL 3, 2017. DOI: 10.1186/s12915-017-0390-6
C Dammers, M Schwarten, AK Buell, D Willbold. Pyroglutamate-modified A beta(3-42) affects aggregation kinetics of A beta(1-42) by accelerating primary and secondary pathways. CHEMICAL SCIENCE, JUL 1, 2017. DOI: 10.1039/c6sc04797a
R Gaspar, G Meisl, AK Buell, L Young, CF Kaminski, TPJ Knowles, E Sparr, S Linse. Secondary nucleation of monomers on fibril surface dominates alpha-synuclein aggregation and provides autocatalytic amyloid amplification. QUARTERLY REVIEWS OF BIOPHYSICS, MAR 20, 2017. DOI: 10.1017/S0033583516000172
R Gaspar, G Meisl, AK Buell, L Young, CF Kaminski, TPJ Knowles, E Sparr, S Linse. Acceleration of alpha-synuclein aggregation. AMYLOID-JOURNAL OF PROTEIN FOLDING DISORDERS, MAR, 2017. DOI: 10.1080/13506129.2017.1292904
AK Buell. The Nucleation of Protein Aggregates – From Crystals to Amyloid Fibrils. EARLY STAGE PROTEIN MISFOLDING AND AMYLOID AGGREGATION, 2017. DOI: 10.1016/bs.ircmb.2016.08.014
2016
JWP Brown, AK Buell, TCT Michaels, G Meisl, J Carozza, P Flagmeier, M Vendruscolo, TPJ Knowles, CM Dobson, C Galvagnion. beta-Synuclein suppresses both the initiation and amplification steps of alpha-synuclein aggregation via competitive binding to surfaces. SCIENTIFIC REPORTS, NOV 3, 2016. DOI: 10.1038/srep36010
Y Zhang, AK Buell, T Muller, E DeGenst, J Benesch, CM Dobson, TPJ Knowles. Protein Aggregate-Ligand Binding Assays Based on Microfluidic Diffusional Separation. CHEMBIOCHEM, OCT 17, 2016. DOI: 10.1002/cbic.201600384
G Costantini, Z Budrikis, A Taloni, AK Buell, S Zapperi, CAM LaPorta. Fluctuations in Protein Aggregation: Design of Preclinical Screening for Early Diagnosis of Neurodegenerative Disease. PHYSICAL REVIEW APPLIED, SEP 21, 2016. DOI: 10.1103/PhysRevApplied.6.034012
P Flagmeier, G Meisl, M Vendruscolo, TPJ Knowles, CM Dobson, AK Buell, C Galvagnion. Mutations associated with familial Parkinson’s disease alter the initiation and amplification steps of alpha-synuclein aggregation. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, SEP 13, 2016. DOI: 10.1073/pnas.1604645113
A Saric, AK Buell, G Meisl, TCT Michaels, CM Dobson, S Linse, TPJ Knowles, D Frenkel. Physical determinants of the self-replication of protein fibrils. NATURE PHYSICS, SEP, 2016. DOI: 10.1038/NPHYS3828
TO Mason, TCT Michaels, A Levin, E Gazit, CM Dobson, AK Buell, TPJ Knowles. Synthesis of Nonequilibrium Supramolecular Peptide Polymers on a Microfluidic Platform. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, AUG 3, 2016. DOI: 10.1021/jacs.6b04136
C Galvagnion, JWP Brown, MM Ouberai, P Flagmeier, M Vendruscolo, AK Buell, E Sparr, CM Dobson. Chemical properties of lipids strongly affect the kinetics of the membrane-induced aggregation of alpha-synuclein. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, JUN 28, 2016. DOI: 10.1073/pnas.1601899113
D Pinotsi, CH Michel, AK Buell, RF Laine, P Mahou, CM Dobson, CF Kaminski, GSK Schierle. Nanoscopic insights into seeding mechanisms and toxicity of alpha-synuclein species in neurons. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, APR 5, 2016. DOI: 10.1073/pnas.1516546113
M Wolff, JJ Mittag, TW Herling, E DeGenst, CM Dobson, TPJ Knowles, D Braun, AK Buell. Quantitative thermophoretic study of disease-related protein aggregates. SCIENTIFIC REPORTS, MAR 17, 2016. DOI: 10.1038/srep22829
2015
TCT Michaels, P Yde, JCW Willis, MH Jensen, D Otzen, CM Dobson, AK Buell, TPJ Knowles. The length distribution of frangible biofilaments. JOURNAL OF CHEMICAL PHYSICS, OCT 28, 2015. DOI: 10.1063/1.4933230
AK Buell, C Galvagnion, CM Dobson, TPJ Knowles. New insights into the mechanism of amyloid formation by alpha-synuclein. EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS, JUL, 2015.
IM vanderWateren, AK Buell, C Galvagnion, EV Yates, CM Dobson. Thioflavin-T: application for alpha-synuclein aggregation kinetics. EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS, JUL, 2015.
C Galvagnion, AK Buell. Fundamental mechanisms of amyloid fibers by the formation of a-synuclein protein in Parkinson’s disease quantitative Modeling. M S-MEDECINE SCIENCES, JUN-JUL, 2015. DOI: 10.1051/medsci/20153106008
C Huynen, N Willet, AK Buell, AS Duwez, C Jerome, M Dumoulin. Influence of the protein context on the polyglutamine length-dependent elongation of amyloid fibrils. BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, MAR, 2015. DOI: 10.1016/j.bbapap.2014.12.002
C Galvagnion, AK Buell, G Meisl, TCT Michaels, M Vendruscolo, TPJ Knowles, CM Dobson. Lipid vesicles trigger alpha-synuclein aggregation by stimulating primary nucleation. NATURE CHEMICAL BIOLOGY, MAR, 2015. DOI: 10.1038/NCHEMBIO.1750
U Shimanovich, I Efimov, TO Mason, P Flagmeier, AK Buell, A Gedanken, S Linse, KS Akerfeldt, CM Dobson, DA Weitz, TPJ Knowles. Protein Microgels from Amyloid Fibril Networks. ACS NANO, JAN, 2015. DOI: 10.1021/nn504869d
2014
A Levin, TO Mason, L Adler-Abramovich, AK Buell, G Meisl, C Galvagnion, Y Bram, SA Stratford, CM Dobson, TPJ Knowles, E Gazit. Ostwald’s rule of stages governs structural transitions and morphology of dipeptide supramolecular polymers. NATURE COMMUNICATIONS, NOV, 2014. DOI: 10.1038/ncomms6219
AK Buell, C Galvagnion, R Gaspar, E Sparr, M Vendruscolo, TPJ Knowles, S Linse, CM Dobson. Solution conditions determine the relative importance of nucleation and growth processes in alpha-synuclein aggregation. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, MAY 27, 2014. DOI: 10.1073/pnas.1315346111
N Lorenzen, SB Nielsen, AK Buell, JD Kaspersen, P Arosio, BS Vad, W Paslawski, G Christiansen, Z Valnickova-Hansen, M Andreasen, JJ Enghild, JS Pedersen, CM Dobson, TPJ Knowles, DE Otzen. The Role of Stable alpha-Synuclein Oligomers in the Molecular Events Underlying Amyloid Formation. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, MAR 12, 2014. DOI: 10.1021/ja411577t
SIA Cohen, L Rajah, CH Yoon, AK Buell, DA White, RA Sperling, M Vendruscolo, EM Terentjev, CM Dobson, DA Weitz, TPJ Knowles. Spatial Propagation of Protein Polymerization. PHYSICAL REVIEW LETTERS, MAR 4, 2014. DOI: 10.1103/PhysRevLett.112.098101
TCT Michaels, AK Buell, EM Terentjev, TPJ Knowles. Quantitative Analysis of Diffusive Reactions at the Solid-Liquid Interface in Finite Systems. JOURNAL OF PHYSICAL CHEMISTRY LETTERS, FEB 20, 2014. DOI: 10.1021/jz4024833
G Toth, SJ Gardai, W Zago, CW Bertoncini, N Cremades, SL Roy, MA Tambe, JC Rochet, C Galvagnion, G Skibinski, S Finkbeiner, M Bova, K Regnstrom, SS Chiou, J Johnston, K Callaway, JP Anderson, MF Jobling, AK Buell, TA Yednock, TPJ Knowles, M Vendruscolo, J Christodoulou, CM Dobson, D Schenk, L McConlogue. Targeting the Intrinsically Disordered Structural Ensemble of alpha-Synuclein by Small Molecules as a Potential Therapeutic Strategy for Parkinson’s Disease. PLOS ONE, FEB 14, 2014. DOI: 10.1371/journal.pone.0087133
TO Mason, DY Chirgadze, A Levin, L Adler-Abramovich, E Gazit, TPJ Knowles, AK Buell. Expanding the Solvent Chemical Space for Self-Assembly of Dipeptide Nanostructures. ACS NANO, FEB, 2014. DOI: 10.1021/nn404237f
FA Aprile, G Meisl, AK Buell, P Flagmeier, CM Dobson, M Vendruscolo, TPJ Knowles. Determination of Primary Nucleation Mechanisms of alpha-Synuclein Amyloid Aggregation. BIOPHYSICAL JOURNAL, JAN 28, 2014.
AK Buell, CM Dobson, TPJ Knowles. The physical chemistry of the amyloid phenomenon: thermodynamics and kinetics of filamentous protein aggregation. AMYLOIDS IN HEALTH AND DISEASE, 2014. DOI: 10.1042/BSE0560011
D Pinotsi, AK Buell, C Galvagnion, CM Dobson, GSK Schierle, CF Kaminski. Direct Observation of Heterogeneous Amyloid Fibril Growth Kinetics via Two-Color Super-Resolution Microscopy. NANO LETTERS, JAN, 2014. DOI: 10.1021/nl4041093
2013
A Pica, A Merlino, AK Buell, TPJ Knowles, E Pizzo, G D’Alessio, F Sica, L Mazzarella. Three-dimensional domain swapping and supramolecular protein assembly: insights from the X-ray structure of a dimeric swapped variant of human pancreatic RNase. ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, OCT, 2013. DOI: 10.1107/S0907444913020507
T Guilliams, F El-Turk, AK Buell, EM O’Day, FA Aprile, EK Esbjorner, M Vendruscolo, N Cremades, E Pardon, L Wyns, ME Welland, J Steyaert, J Christodoulou, CM Dobson, E DeGenst. Nanobodies Raised against Monomeric alpha-Synuclein Distinguish between Fibrils at Different Maturation Stages. JOURNAL OF MOLECULAR BIOLOGY, JUL 24, 2013. DOI: 10.1016/j.jmb.2013.01.040
D Pinotsi, AK Buell, CM Dobson, GSK Schierle, CF Kaminski. A Label-Free, Quantitative Assay of Amyloid Fibril Growth Based on Intrinsic Fluorescence. CHEMBIOCHEM, MAY 10, 2013. DOI: 10.1002/cbic.201300103
LQ Xu, S Wu, AK Buell, SIA Cohen, LJ Chen, WH Hu, SA Cusack, LS Itzhaki, H Zhang, TPJ Knowles, CM Dobson, ME Welland, GW Jones, S Perrett. Influence of specific HSP70 domains on fibril formation of the yeast prion protein Ure2. PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, MAY 5, 2013. DOI: 10.1098/rstb.2011.0410
AK Buell, P Hung, X Salvatella, ME Welland, CM Dobson, TPJ Knowles. Electrostatic Effects in Filamentous Protein Aggregation. BIOPHYSICAL JOURNAL, MAR 5, 2013. DOI: 10.1016/j.bpj.2013.01.031
2012
C Roodveldt, A Andersson, EJ DeGenst, A Labrador-Garrido, AK Buell, CM Dobson, GG Tartaglia, M Vendruscolo. A Rationally Designed Six-Residue Swap Generates Comparability in the Aggregation Behavior of alpha-Synuclein and beta-Synuclein. BIOCHEMISTRY, NOV 6, 2012. DOI: 10.1021/bi300558q
AK Buell, CM Dobson, ME Welland. Measuring the Kinetics of Amyloid Fibril Elongation Using Quartz Crystal Microbalances. AMYLOID PROTEINS: METHODS AND PROTOCOLS, SECOND EDITION, 2012. DOI: 10.1007/978-1-61779-551-0_8
AK Buell, A Dhulesia, DA White, TPJ Knowles, CM Dobson, ME Welland. Detailed Analysis of the Energy Barriers for Amyloid Fibril Growth. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2012. DOI: 10.1002/anie.201108040
2011
SL Shammas, CA Waudby, SY Wang, AK Buell, TPJ Knowles, H Ecroyd, ME Welland, JA Carver, CM Dobson, S Meehan. Binding of the Molecular Chaperone alpha B-Crystallin to A beta Amyloid Fibrils Inhibits Fibril Elongation. BIOPHYSICAL JOURNAL, OCT 5, 2011. DOI: 10.1016/j.bpj.2011.07.056
AK Buell, A Dhulesia, MF Mossuto, N Cremades, JR Kumita, M Dumoulin, ME Welland, TPJ Knowles, X Salvatella, CM Dobson. Population of Nonnative States of Lysozyme Variants Drives Amyloid Fibril Formation. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, MAY 25, 2011. DOI: 10.1021/ja109620d
YQ Wang, AK Buell, XY Wang, ME Welland, CM Dobson, TPJ Knowles, S Perrett. Relationship between Prion Propensity and the Rates of Individual Molecular Steps of Fibril Assembly. JOURNAL OF BIOLOGICAL CHEMISTRY, APR 8, 2011. DOI: 10.1074/jbc.M110.208934
AK Buell, EK Esbjorner, PJ Riss, DA White, FI Aigbirhio, G Toth, ME Welland, CM Dobson, TPJ Knowles. Probing small molecule binding to amyloid fibrils. PHYSICAL CHEMISTRY CHEMICAL PHYSICS, 2011. DOI: 10.1039/c1cp22283j
2010
AK Buell, CM Dobson, TPJ Knowles, ME Welland. Interactions between Amyloidophilic Dyes and Their Relevance to Studies of Amyloid Inhibitors. BIOPHYSICAL JOURNAL, NOV 17, 2010. DOI: 10.1016/j.bpj.2010.08.074
AK Buell, DA White, C Meier, ME Welland, TPJ Knowles, CM Dobson. Surface Attachment of Protein Fibrils via Covalent Modification Strategies. JOURNAL OF PHYSICAL CHEMISTRY B, SEP 2, 2010. DOI: 10.1021/jp101579n
AK Buell, JR Blundell, CM Dobson, ME Welland, EM Terentjev, TPJ Knowles. Frequency Factors in a Landscape Model of Filamentous Protein Aggregation. PHYSICAL REVIEW LETTERS, JUN 1, 2010. DOI: 10.1103/PhysRevLett.104.228101
DA White, AK Buell, TPJ Knowles, ME Welland, CM Dobson. Protein Aggregation in Crowded Environments. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, APR 14, 2010. DOI: 10.1021/ja909997e
TPJ Knowles, TW Oppenheim, AK Buell, DY Chirgadze, ME Welland. Nanostructured films from hierarchical self-assembly of amyloidogenic proteins. NATURE NANOTECHNOLOGY, MAR, 2010. DOI: 10.1038/NNANO.2010.26
2009
DA White, AK Buell, CM Dobson, ME Welland, TPJ Knowles. Biosensor-based label-free assays of amyloid growth. FEBS LETTERS, AUG 20, 2009. DOI: 10.1016/j.febslet.2009.06.008
AK Buell, GG Tartaglia, NR Birkett, CA Waudby, M Vendruscolo, X Salvatella, ME Welland, CM Dobson, TPJ Knowles. Position-Dependent Electrostatic Protection against Protein Aggregation. CHEMBIOCHEM, MAY 25, 2009. DOI: 10.1002/cbic.200900144
2008
WS Hopkins, HP Loock, B Cronin, MGD Nix, AL Devine, RN Dixon, MNR Ashfold, HM Yin, SJ Rowling, A Bull, SH Kable. Quantitative (v, N, K(a)) product state distributions near the triplet threshold for the reaction H(2)CO -> H+HCO measured by Rydberg tagging and laser-induced fluorescence. JOURNAL OF PHYSICAL CHEMISTRY A, OCT 2, 2008. DOI: 10.1021/jp8021826
2007
HM Yin, SJ Rowling, A Bull, SH Kable. Photodissociation dynamics of the reaction H(2)CO -> H+HCO via the singlet (S(0)) and triplet (T(1)) surfaces. JOURNAL OF CHEMICAL PHYSICS, AUG 14, 2007. DOI: 10.1063/1.2752161
Protein Biophysics at DTU Bioengineering 